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anti tfeb  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti tfeb
    Anti Tfeb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tfeb/TFEB+Rabbit+mAb/pm41912102-148-9-14
    Average 95 stars, based on 123 article reviews
    anti tfeb - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: GATOR1 complex controls cisplatin sensitivity
    Article Snippet: Anti-NPRL2 (37344), anti-CTR1 (13086), anti-phospho-p70 S6 kinase (Thr389) (9206), anti-p70 S6 kinase (9202), anti-p4EBP1 (Thr37/46) (2855), anti-4EBP1 (9452), anti-pTFEB (Ser211) (37681), anti-TFEB (37785), anti-pULK1 (Ser757) (14202), anti-ULK1 (8054), anti-pS6 (Ser 235/236) (2211), anti-S6 (2317), anti-p62 (8025), anti-phospho-histone-H2AX Ser139/Tyr142 (5438), anti-PARP1 (9542), anti-pAKT Ser473 (4060), anti-AKT (4619), anti-pAMPKα (Thr172) (2535), anti-AMPKα (5831), anti-phospho-p53 (Ser15) (9284), anti-p53 (2524), anti-pATM S1981 (4526), anti-ATM (2873), anti-ATR (2790), anti-pCHK1 (S345) (2341), anti-CHK1 (2360), anti-pCHK2 (Thr68) (2661), anti-CHK2 (2662), anti-p21 (2946), anti-LC3B (2775), anti-caspase 3 (9662), anti-GAPDH (2118), anti-vinculin (18799) were from Cell Signaling Technology.

    Article Title: Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β-TFEB signaling axis.
    Article Snippet: Primary antibodies used in this study were as follows: anti-TFEB (37785, 1:1000; 91767, 1:100; Cell Signaling Technology), antiLAMP2 (34141, 1:1000; Cell Signaling Technology), anti-cathepsin B (31718, 1:1000; Cell Signaling Technology), anti-cathepsin D (88239.

    Blocking Assay:

    Article Title: Legionella Lem26 functions as an ATG8-activated effector that inhibits host autophagy
    Article Snippet: The following day, plasmids were either transfected (Mirus TransIT-LT1) for 24 h and/or starved for 2–4 h prior to fixation in 4% PFA (Electron Microscopy Sciences 15710). .. Cells were permeabilized in blocking solution (PBS containing 0.05% saponin; 2% BSA) for 30 min, followed by a 1 h incubation with primary antibody: anti-ubiquitin (FK2) (Enzo Life Sciences BML-PW8810, 1:100), anti-p62 (BD Biosciences 610832, 1:300), anti-LC3 (MBL International PM036, 1:500), ATG9A (Abcam ab108338, 1:250), and anti-TFEB (Cell Signaling #37785, 1:100). ..

    Article Title: Legionella Lem26 functions as an ATG8-activated effector that inhibits host autophagy.
    Article Snippet: The following day, plasmids were either transfected (Mirus TransIT-LT1) for 24 h and/or starved for 2–4 h prior to fixation in 4% PFA (Electron Microscopy Sciences 15710). .. Cells were permeabilized in blocking solution (PBS containing 0.05% saponin; 2% BSA) for 30 min, followed by a 1 h incubation with primary antibody: anti-ubiqui tin (FK2) (Enzo Life Sciences BML-PW8810, 1:100), anti-p62 (BD Biosciences 610832, 1:300), anti-LC3 (MBL International PM036, 1:500), ATG9A (Abcam ab108338, 1:250), and anti-TFEB (Cell Signaling #37785, 1:100). ..

    Article Title: ATM-dependent RHEB phosphorylation couples DNA damage to lysosomal mTORC1 signaling to orchestrate the cellular response to genotoxic stress
    Article Snippet: .. Staining with anti-TFEB (#4240, Cell Signaling Technology) and anti-γH2AX (#05-636, Sigma) antibodies (1:200 and 1:500, respectively in blocking buffer) was performed by incubation for 16 h at 4 °C. .. After staining with primary antibodies, coverslips were washed three times with PBS and then stained with highly cross-adsorbed fluorescent secondary antibodies (Donkey anti-Rabbit Alexa Fluor 488, Donkey anti-Mouse Alexa Fluor 594; both from Jackson ImmunoResearch) diluted 1:400 in blocking buffer for 1 hour at room temperature.

    Incubation:

    Article Title: Legionella Lem26 functions as an ATG8-activated effector that inhibits host autophagy
    Article Snippet: The following day, plasmids were either transfected (Mirus TransIT-LT1) for 24 h and/or starved for 2–4 h prior to fixation in 4% PFA (Electron Microscopy Sciences 15710). .. Cells were permeabilized in blocking solution (PBS containing 0.05% saponin; 2% BSA) for 30 min, followed by a 1 h incubation with primary antibody: anti-ubiquitin (FK2) (Enzo Life Sciences BML-PW8810, 1:100), anti-p62 (BD Biosciences 610832, 1:300), anti-LC3 (MBL International PM036, 1:500), ATG9A (Abcam ab108338, 1:250), and anti-TFEB (Cell Signaling #37785, 1:100). ..

    Article Title: Legionella Lem26 functions as an ATG8-activated effector that inhibits host autophagy.
    Article Snippet: The following day, plasmids were either transfected (Mirus TransIT-LT1) for 24 h and/or starved for 2–4 h prior to fixation in 4% PFA (Electron Microscopy Sciences 15710). .. Cells were permeabilized in blocking solution (PBS containing 0.05% saponin; 2% BSA) for 30 min, followed by a 1 h incubation with primary antibody: anti-ubiqui tin (FK2) (Enzo Life Sciences BML-PW8810, 1:100), anti-p62 (BD Biosciences 610832, 1:300), anti-LC3 (MBL International PM036, 1:500), ATG9A (Abcam ab108338, 1:250), and anti-TFEB (Cell Signaling #37785, 1:100). ..

    Article Title: ATM-dependent RHEB phosphorylation couples DNA damage to lysosomal mTORC1 signaling to orchestrate the cellular response to genotoxic stress
    Article Snippet: .. Staining with anti-TFEB (#4240, Cell Signaling Technology) and anti-γH2AX (#05-636, Sigma) antibodies (1:200 and 1:500, respectively in blocking buffer) was performed by incubation for 16 h at 4 °C. .. After staining with primary antibodies, coverslips were washed three times with PBS and then stained with highly cross-adsorbed fluorescent secondary antibodies (Donkey anti-Rabbit Alexa Fluor 488, Donkey anti-Mouse Alexa Fluor 594; both from Jackson ImmunoResearch) diluted 1:400 in blocking buffer for 1 hour at room temperature.

    Western Blot:

    Article Title: Melatonin ameliorates sepsis-induced myocardial dysfunction by promoting autophagy via SIRT1-mediated TFEB deacetylation.
    Article Snippet: Sepsis-induced myocardial dysfunction (SIMD) is an acute cardiac dysfunction syndrome associated with sepsis but independent of ischemia, serving as a critical contributor to cardiogenic shock, refractory shock and mortality.. Current clinical treatments remain far from achieving satisfactory outcomes.. Our previous study found that serum melatonin levels were significantly lower in septic patients compared to healthy volunteers.

    Staining:

    Article Title: ATM-dependent RHEB phosphorylation couples DNA damage to lysosomal mTORC1 signaling to orchestrate the cellular response to genotoxic stress
    Article Snippet: .. Staining with anti-TFEB (#4240, Cell Signaling Technology) and anti-γH2AX (#05-636, Sigma) antibodies (1:200 and 1:500, respectively in blocking buffer) was performed by incubation for 16 h at 4 °C. .. After staining with primary antibodies, coverslips were washed three times with PBS and then stained with highly cross-adsorbed fluorescent secondary antibodies (Donkey anti-Rabbit Alexa Fluor 488, Donkey anti-Mouse Alexa Fluor 594; both from Jackson ImmunoResearch) diluted 1:400 in blocking buffer for 1 hour at room temperature.



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